Analytical Sciences, Talk
Electrostatic Spray Ionization Mass Spectrometry for Biochemical Analysis and Imaging
Liang Qiao1, Hubert Girault1
1Laboratoire d’Electrochimie Physique et Analytique, EPFL, Lausanne
Electrostatic spray ionization (ESTASI) mass spectrometry (MS) is a new ambient ionization MS technique developed in our group.1 During ESTASI, a sample in electrolyte solution is placed on an insulating plate, and an electrode is placed under the insulating plate. Bipolar spray ionization of samples happens under the application of a pulsed square-wave high voltage between the electrode and MS ion entrance.
Since the invention of ESTASI, we have mainly applied it in (1) interfacing capillary electrophoresis (CE) and MS,1 (2) interfacing gel electrophoresis and MS,2 (3) ambient ionization MS for direct analysis of samples with minimal preparation,3 and (4) MS imaging4. The ESTASI is a versatile ambient ionization technique in biochemical analysis.
[1] L. Qiao, R. Sartor, N. Gasilova, Y. Lu, E. Tobolkina, B. Liu, H. Girault, Anal. Chem. 2012, 84, 7422.
[2] L. Qiao, E. Tobolkina, B. Liu, H. Girault, Anal. Chem. 2013, 85, 4745.
[3] E. Tobolkina, L. Qiao, G. Xu, H. Girault, Rapid Commun. Mass Spectrom. 2013, 27, 2310.
[4] L. Qiao, E. Tobolkina, A. Lesch, A. Bondarenko, X. Zhong, B. Liu, H. Pick, H. Vogel, H. Girault, Anal. Chem. 2014, 86, 2033.